Entry 1 sense Webster, 1913 Amputation /ămˌ-pyət-āsh'-ən/ · Am·pu·ta·tion · IPA /ˌæmpjʊˈteɪʃən/ 01 n. The act of amputating; esp. the operation of cutting off a limb or projecting part of the body. 1. The act of amputating; esp. the operation of cutting off a limb or projecting part of the body.